Validation of a hybrid approach to standardize immunophenotyping analysis in large population studies: The Health and Retirement Study.

ABSTRACT: Traditional manual gating strategies are often time-intensive, place a high burden on the analyzer, and are susceptible to bias between analyzers. Several automated gating methods have shown to exceed performance of manual gating for a limited number of cell subsets. However, many of the automated algorithms still require significant manual interventions or have yet to demonstrate their utility in large datasets. Therefore, we developed an approach that utilizes a previously published automated algorithm (OpenCyto framework) with a manually created hierarchically cell gating template implemented, along with a custom developed visualization software (FlowAnnotator) to rapidly and efficiently analyze immunophenotyping data in large population studies. This approach allows pre-defining popula

SUBMITTER: Hunter-Schlichting D 

PROVIDER: S-EPMC7260195 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature