Dual role of Ca²⁺-activated Cl^- channel transmembrane member 16A in lipopolysaccharide-induced intestinal epithelial barrier dysfunction in vitro.

ABSTRACT: Dysfunction of intestinal epithelial Cl^- currents and channels have previously been reported in inflammatory intestinal diseases. However, the expression and function of the newly identified Ca²⁺-activated Cl^- channel transmembrane member 16A (TMEM16A) in the intestinal epithelium is unclear. In this study, we investigated the effects of TMEM16A on intestinal epithelial barrier function in vitro. Intestinal epithelial barrier dysfunction was modeled by lipopolysaccharide (LPS)-induced cell damage in intestinal epithelial IEC-6 cells and the effects of TMEM16A knockdown and overexpression on cell apoptosis and tight junctions were studied. Corresponding mRNA and protein expression levels were measured by quantitative real-time polymerase chain reaction, west

SUBMITTER: Sui J 

PROVIDER: S-EPMC7260209 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature