Identification and characterization of SSR markers of Guadua chacoensis (Rojas) Londono & P.M. Peterson and transferability to other bamboo species.

ABSTRACT: The aim of this study was to develop simple sequence repeat (SSR) markers for genetic studies on G. chacoensis, as well as to evaluate their transferability to other bamboo species. Genomic DNA was isolated from G. chacoensis and its partial sequencing was used to find SSR loci. The obtained sequencing data were de novo assembled using the software CLC Genomics Workbench^® 8.0v. The SSR loci primers were identified and designed with the software SSRLocator. The selected markers were validated using 56 plants sampled in seven populations from southern Brazil. The markers with potential polymorphism were selected and fluorescently labeled for characterization by capillary electrophoresis. In total, 92 SSR loci were found in G. chacoensis contigs. Suitable prime

SUBMITTER: Rossarolla MD 

PROVIDER: S-EPMC7260311 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature