Vitrified Human Umbilical Arteries as Potential Grafts for Vascular Tissue Engineering.

ABSTRACT:

Background

The development of a biological based small diameter vascular graft (d < 6 mm), that can be properly stored over a long time period at - 196 °C, in order to directly be used to the patients, still remains a challenge. In this study the decellularized umbilical arteries (UAs) where vitrified, evaluated their composition and implanted to a porcine model, thus serving as vascular graft.

Methods

Human UAs were decellularized using 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and sodium dodecyl sulfate (SDS) detergents. Then, vitrified with vitrification solution 55 (VS55) solution, remained for 6 months in liquid nitrogen and their extracellular matrix composition was compared to conventionally cryopreserved UAs. Additionally, total hydroxyproline

SUBMITTER: Mallis P 

PROVIDER: S-EPMC7260347 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature