Development of MicroRNA-146a-Enriched Stem Cell Secretome for Wound-Healing Applications.

ABSTRACT: Secretome-based therapies have the potential to become the next generation of viable therapeutic wound repair treatments. However, precise strategies aimed to refine and control the secretome composition are necessary to enhance its therapeutic efficacy and facilitate clinical translation. In this study, we aim to accomplish this by transfecting human adipose-derived stem cells (hASCs) with microRNA-146a, which is a potent regulator of angiogenesis and inflammation. The secretome composition obtained from the transfected hASCs (secretome^146a) was characterized and compared to nontransfected hASCs secretome to evaluate changes in angiogenic and anti-inflammatory growth factor, cytokine, and miRNA content. In vitro proliferation, migration, and tubular morphogenesis assays using h

SUBMITTER: Waters R 

PROVIDER: S-EPMC7260687 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature