Escherichia coli metabolism under short-term repetitive substrate dynamics: adaptation and trade-offs.

ABSTRACT:

Background

Microbial metabolism is highly dependent on the environmental conditions. Especially, the substrate concentration, as well as oxygen availability, determine the metabolic rates. In large-scale bioreactors, microorganisms encounter dynamic conditions in substrate and oxygen availability (mixing limitations), which influence their metabolism and subsequently their physiology. Earlier, single substrate pulse experiments were not able to explain the observed physiological changes generated under large-scale industrial fermentation conditions.

Results

In this study we applied a repetitive feast-famine regime in an aerobic Escherichia coli culture in a time-scale of seconds. The regime was applied for several generations, allowing cells to adapt to the (repetitive) dyna

SUBMITTER: Vasilakou E 

PROVIDER: S-EPMC7260802 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature