Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts.

ABSTRACT:

Background

In the poultry and livestock industries, precise genetic information is crucial for improving economic traits. Thus, functional genomic studies help to generate faster, healthier, and more efficient animal production. Chicken myoblast cells, which are required for muscle development and regeneration, are particularly important because chicken growth is closely related to muscle mass.

Results

In this study, we induced expression of microRNA-146b-5p mediated by the piggyBac transposon system in primary chicken myoblast (pCM) cells. Subsequently, we analyzed and compared the proliferation and differentiation capacity and also examined the expression of related genes in regular pCM (rpCM) cells and pCM cells overexpressing miRNA-146b-5p (pCM-146b OE cells). pCM-146b O

SUBMITTER: Lee JH 

PROVIDER: S-EPMC7260857 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature