Endogenous oxidized DNA bases and APE1 regulate the formation of G-quadruplex structures in the genome.

ABSTRACT: Formation of G-quadruplex (G4) DNA structures in key regulatory regions in the genome has emerged as a secondary structure-based epigenetic mechanism for regulating multiple biological processes including transcription, replication, and telomere maintenance. G4 formation (folding), stabilization, and unfolding must be regulated to coordinate G4-mediated biological functions; however, how cells regulate the spatiotemporal formation of G4 structures in the genome is largely unknown. Here, we demonstrate that endogenous oxidized guanine bases in G4 sequences and the subsequent activation of the base excision repair (BER) pathway drive the spatiotemporal formation of G4 structures in the genome. Genome-wide mapping of occurrence of Apurinic/apyrimidinic (AP) site damage, binding of BER protein

SUBMITTER: Roychoudhury S 

PROVIDER: S-EPMC7260947 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature