Light-Inducible Generation of Membrane Curvature in Live Cells with Engineered BAR Domain Proteins.

ABSTRACT: Nanoscale membrane curvature is now understood to play an active role in essential cellular processes such as endocytosis, exocytosis, and actin dynamics. Previous studies have shown that membrane curvature can directly affect protein function and intracellular signaling. However, few methods are able to precisely manipulate membrane curvature in live cells. Here, we report the development of a new method of generating nanoscale membrane curvature in live cells that is controllable, reversible, and capable of precise spatial and temporal manipulation. For this purpose, we make use of Bin/Amphiphysin/Rvs (BAR) domain proteins, a family of well-studied membrane-remodeling and membrane-sculpting proteins. Specifically, we engineered two optogenetic systems, opto-FBAR and opto-IBAR, that allow

SUBMITTER: Jones T 

PROVIDER: S-EPMC7261005 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature