Rapid identification of melioidosis agent by an insulated isothermal PCR on a field-deployable device.

ABSTRACT:

Background

Burkholderia pseudomallei causes melioidosis, a serious illness that can be fatal if untreated or misdiagnosed. Culture from clinical specimens remains the gold standard but has low diagnostic sensitivity.

Method

In this study, we developed a rapid, sensitive and specific insulated isothermal Polymerase Chain Reaction (iiPCR) targeting bimA gene (Burkholderia Intracellular Motility A; BPSS1492) for the identification of B. pseudomallei. A pair of novel primers: BimA(F) and BimA(R) together with a probe were designed and 121 clinical B. pseudomallei strains obtained from numerous clinical sources and 10 ATCC non-targeted strains were tested with iiPCR and qPCR in parallel.

Results

All 121 B. pseudomallei isolates wer

SUBMITTER: Chua KH 

PROVIDER: S-EPMC7261116 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature