Changes of liver transcriptome profiles following oxidative stress in streptozotocin-induced diabetes in mice.

ABSTRACT:

Background

Oxidative-stress (OS) was causal in the development of cell dysfunction and insulin resistance. Streptozotocin (STZ) was an alkylation agent that increased reactive oxygen species (ROS) levels. Here we aimed to explore the oxidative-stress and related RNAs in the liver of STZ-induced diabetic mice.

Methods

RNA-sequencing was performed using liver tissues from STZ induced diabetic mice and controls. Pathway and Gene Ontology (GO) analyses were utilized to annotate the target genes. The differentially expressed RNAs involved in the peroxisome pathway were validated by qRT-PCR. The glucose metabolite and OS markers were measured in the normal control (NC) and STZ-induced diabetic mellitus (DM) group.

Results

The levels of serum Fasting insulin, HbA1c, Malondia

SUBMITTER: Guo S 

PROVIDER: S-EPMC7261117 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature