MiR-125 inhibited cervical cancer progression by regulating VEGF and PI3K/AKT signaling pathway.

ABSTRACT:

Background

MiR-125 has been shown to be involved in a variety of cancers, including cervical cancer (CC). Here, our goal was to explore miR-125 functional role and molecular mechanism in cervical cancer development and progression.

Methods

qRT-PCR was employ to detect miR-125 and VEGF mRNA expression. Western blot was applied for testing protein levels (VEGF, E-cadherin, N-cadherin, vimentin, AKT, p-AKT, PI3K, and p-PI3K). MTT and transwell assays were used for detecting cervical cancer cell progression, including cell viability, migration, and invasion.

Results

We observed that miR-125 was downregulated, whereas VEGF was upregulated in cervical cancer tissues and cell lines (CaSki and SiHa). MiR-125 inhibited the proliferation, invasion, and migration by targeting VE

SUBMITTER: Fu K 

PROVIDER: S-EPMC7261381 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature