Dataset Information

MiR-125 inhibited cervical cancer progression by regulating VEGF and PI3K/AKT signaling pathway.

ABSTRACT:

Background

MiR-125 has been shown to be involved in a variety of cancers, including cervical cancer (CC). Here, our goal was to explore miR-125 functional role and molecular mechanism in cervical cancer development and progression.

Methods

qRT-PCR was employ to detect miR-125 and VEGF mRNA expression. Western blot was applied for testing protein levels (VEGF, E-cadherin, N-cadherin, vimentin, AKT, p-AKT, PI3K, and p-PI3K). MTT and transwell assays were used for detecting cervical cancer cell progression, including cell viability, migration, and invasion.

Results

We observed that miR-125 was downregulated, whereas VEGF was upregulated in cervical cancer tissues and cell lines (CaSki and SiHa). MiR-125 inhibited the proliferation, invasion, and migration by targeting VEGF in cervical cancer. Moreover, miR-125 negatively regulated VEGF expression in cervical cancer tissues. Finally, we demonstrated that miR-520d-5p inhibited the activation of PI3K/AKT signaling pathway.

Conclusion

In conclusion, the findings demonstrated that miR-125 inhibited cervical cancer progression and development by suppression VEGF and PI3K/AKT signaling pathway.

SUBMITTER: Fu K

PROVIDER: S-EPMC7261381 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Publications

MiR-125 inhibited cervical cancer progression by regulating VEGF and PI3K/AKT signaling pathway.

Fu Ke K Zhang Ling L Liu Rui R Shi Qi Q Li Xue X Wang Min M

World journal of surgical oncology 20200530 1

<h4>Background</h4>MiR-125 has been shown to be involved in a variety of cancers, including cervical cancer (CC). Here, our goal was to explore miR-125 functional role and molecular mechanism in cervical cancer development and progression.<h4>Methods</h4>qRT-PCR was employ to detect miR-125 and VEGF mRNA expression. Western blot was applied for testing protein levels (VEGF, E-cadherin, N-cadherin, vimentin, AKT, p-AKT, PI3K, and p-PI3K). MTT and transwell assays were used for detecting cervical ...[more]

PMID: 32473637

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Dataset Information

MiR-125 inhibited cervical cancer progression by regulating VEGF and PI3K/AKT signaling pathway.

Background

Methods

Results

Conclusion

Publications

MiR-125 inhibited cervical cancer progression by regulating VEGF and PI3K/AKT signaling pathway.

Similar Datasets

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