Saccharomyces cerevisiae ER membrane protein complex subunit 4 (EMC4) plays a crucial role in eIF2B-mediated translation regulation and survival under stress conditions.

ABSTRACT: BACKGROUND:Eukaryotic initiation factor 2B (eIF2B) initiates and regulates translation initiation in eukaryotes. eIF2B gene mutations cause leukoencephalopathy called vanishing white matter disease (VWM) in humans and slow growth (Slg-) and general control derepression (Gcd-) phenotypes in Saccharomyces cerevisiae. RESULTS:To suppress eIF2B mutations, S. cerevisiae genomic DNA library was constructed in high-copy vector (YEp24) and transformed into eIF2B mutant S. cerevisiae strains. The library was screened for wild-type genes rescuing S. cerevisiae (Slg-) and (Gcd-) phenotypes. A genomic clone, Suppressor-I (Sup-I), rescued S. cerevisiae Slg- and Gcd- phenotypes (gcd7-201 gcn2∆). The YEp24/Sup-I construct contained truncated TAN1, full length EMC4, full length YGL230C, and truncated SAP4

SUBMITTER: Sharma S 

PROVIDER: S-EPMC7261713 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature