Neuroprotective effects of FK866 against traumatic brain injury: Involvement of p38/ERK pathway.

ABSTRACT:

Objective

FK866 is an inhibitor of nicotinamide phosphoribosyltransferase (NAMPT), which exhibits neuroprotective effects in ischemic brain injury. However, in traumatic brain injury (TBI), the role and mechanism of FK866 remain unclear. The present research was aimed to investigate whether FK866 could attenuate TBI and clarified the underlying mechanisms.

Methods

A controlled cortical impact model was established, and FK866 at a dose of 5 mg/kg was administered intraperitoneally at 1 h and 6 h, then twice per day post-TBI until sacrifice. Brain water content, Evans blue dye extravasation, modified neurological severity scores (mNSS), Morris water maze test, enzyme-linked immunosorbent assay (ELISA), immunofluorescence staining, and western blot were performed.

Results

SUBMITTER: Tan Z 

PROVIDER: S-EPMC7261767 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature