Investigation of Inter- and Intratumoral Heterogeneity of Glioblastoma Using TOF-SIMS.

ABSTRACT: Glioblastoma (GBM) is one of the most aggressive human cancers with a median survival of less than two years. A distinguishing pathological feature of GBM is a high degree of inter- and intratumoral heterogeneity. Intertumoral heterogeneity of GBM has been extensively investigated on genomic, methylomic, transcriptomic, proteomic and metabolomics levels, however only a few studies describe intratumoral heterogeneity because of the lack of methods allowing to analyze GBM samples with high spatial resolution. Here, we applied TOF-SIMS (Time-of-flight secondary ion mass spectrometry) for the analysis of single cells and clinical samples such as paraffin and frozen tumor sections obtained from 57 patients. We developed a technique that allows us to simultaneously detect the distribution of pro

SUBMITTER: Gularyan SK 

PROVIDER: S-EPMC7261812 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature