DEqMS: A Method for Accurate Variance Estimation in Differential Protein Expression Analysis.

ABSTRACT: Quantitative proteomics by mass spectrometry is widely used in biomarker research and basic biology research for investigation of phenotype level cellular events. Despite the wide application, the methodology for statistical analysis of differentially expressed proteins has not been unified. Various methods such as t test, linear model and mixed effect models are used to define changes in proteomics experiments. However, none of these methods consider the specific structure of MS-data. Choices between methods, often originally developed for other types of data, are based on compromises between features such as statistical power, general applicability and user friendliness. Furthermore, whether to include proteins identified with one peptide in statistical analysis of differential pr

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC7261819 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature