Single-Cell RNA Analysis of Type I Spiral Ganglion Neurons Reveals a <i>Lmx1a</i> Population in the Cochlea.

ABSTRACT: In the mature cochlea, each inner hair cell (IHC) is innervated by multiple spiral ganglion neurons of type I (SGNI). SGNIs are morphologically and electro-physiologically diverse. Also, they differ in their susceptibility to noise insult. However, the molecular underpinnings of their identity and physiological differences remain poorly understood. In this study, we developed a novel triple transgenic mouse, which enabled the isolation of pure populations of SGNIs and the analysis of a 96-gene panel via single-cell qPCR. We found three distinct populations of Type I SGNs, which were marked by their exclusive expression of Lmx1a, Slc4a4, or Mfap4/Fzd2, respectively, at postnatal days P3, P8, and P12. Our data suggest that afferent SGN subtypes are established geneticall

SUBMITTER: Grandi FC 

PROVIDER: S-EPMC7261882 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature