An Engineered Mouse to Identify Proliferating Cells and Their Derivatives.

ABSTRACT:

Background

Cell proliferation is a fundamental event during development, disease, and regeneration. Effectively tracking and quantifying proliferating cells and their derivatives is critical for addressing many research questions. Cell cycle expression such as for Ki67, proliferating cell nuclear antigen (PCNA), or aurora kinase B (Aurkb), or measurement of 5-bromo-2'-deoxyuridine (BrdU) or ³H-thymidine incorporation have been widely used to assess and quantify cell proliferation. These are powerful tools for detecting actively proliferating cells, but they do not identify cell populations derived from proliferating progenitors over time.

Aims

We developed a new mouse tool for lineage tracing of proliferating cells by targeting the Aurkb allele.

Results<

SUBMITTER: Jang J 

PROVIDER: S-EPMC7261916 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature