Apparent attenuation by opto-acoustic defocus in phonon microscopy.

ABSTRACT: Understanding the mechanical properties of biological cells is a challenging problem for the life sciences partly because there are limited methods for mapping elasticity with high resolution. Phonon microscopy is a form of Brillouin light scattering which uses coherent phonons for imaging with elasticity-related contrast, phonon resolution and without labels. It can measure material properties such as sound velocity, acoustic impedance and attenuation. To use it as a contrast mechanism in microscopy, high numerical aperture (NA) lenses are key to high resolution. However, increasing NA induces apparent attenuation, a premature decay of the detected signal. To reduce signal decay and quantify the sound attenuation coefficient in cells, it is necessary to understand the mechanisms that affe

SUBMITTER: Perez-Cota F 

PROVIDER: S-EPMC7262445 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature