Autophagy Regulates Fungal Virulence and Sexual Reproduction in Cryptococcus neoformans.

ABSTRACT: Autophagy (macroautophagy) is an evolutionarily conserved degradation pathway involved in bulk degradation of cytoplasmic organelles, old protein, and other macromolecules and nutrient recycling during starvation. Extensive studies on functions of autophagy-related genes have revealed that autophagy plays a role in cell differentiation and pathogenesis of pathogenic fungi. In this study, we identified and characterized 14 core autophagy machinery genes (ATGs) in C. neoformans. To understand the function of autophagy in virulence and fungal development in C. neoformans, we knocked out the 14 ATGs in both α and a mating type strain backgrounds in C. neoformans, respectively, by using biolistic transformation and in vivo homologous recombinatio

SUBMITTER: Jiang ST 

PROVIDER: S-EPMC7262457 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature