Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes.

ABSTRACT:

Background

Extracellular vesicles (EVs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. The aim of the present report was to provide the first isolation, quantification and protein characterization of EVs from buffalo (Bubalus bubalis) sera infected with Theileria spp.

Methods

Infected animals were identified through optical microscopy and PCR. EVs were isolated from buffalo sera by size-exclusion chromatography and characterized using western blotting analysis, nanoparticle tracking analysis and transmission electron microscopy. Subsequently, the proteins from isolated vesicles were characterized by mass spectrometry.

Results

EVs from buffalo sera have shown sizes in the 124-140 nm range and 306 proteins were characteri

SUBMITTER: de Pontes LG 

PROVIDER: S-EPMC7262785 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature