Cytochrome P450 metabolism mediates low-temperature resistance in pinewood nematode.

ABSTRACT: Pinewood nematode (PWN; Bursaphelenchus xylophilus) is a devastating invasive species that is expanding into colder regions. Here, we investigated the molecular mechanisms underlying low-temperature resistance of PWN. We identified differentially expressed genes enriched under low temperature in previously published transcriptome data using the Kyoto Encyclopedia of Genes and Genomes. Quantitative real-time PCR was used to further validate the transcript level changes of three known cytochrome P450 genes under low temperature. RNA interference was used to validate the low-temperature resistance function of three cytochrome P450 genes from PWN. We report that differentially expressed genes were significantly enriched in two cytochrome P450-related pathways under low-temperature treatment. H

SUBMITTER: Wang B 

PROVIDER: S-EPMC7262887 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature