Serum Folate Forms Are Stable during Repeated Analysis in the Presence of Ascorbic Acid and during Frozen Sample Storage.

ABSTRACT:

Background

Serum folate forms, and particularly tetrahydrofolate, are sensitive to oxidation.

Methods

Using a repeated measures design, we investigated the stability of folate forms in convenience samples with added ascorbic acid (AA; 5 g/L) analyzed initially and after variable (approximately 1-33 weeks) storage time at -70 °C. We examined the recovery of tetrahydrofolate added at different spiking levels to serum with and without AA (5 g/L). We also assessed the long-term frozen storage stability of folate forms.

Results

Repeat analysis produced consistent results with the initial analysis; the mean relative change (95% CI; Lin's concordance correlation between initial and repeat result; sample size) was 0.08% (-0.24% to 0.39%; r _c = 0.999;

SUBMITTER: Paladugula N 

PROVIDER: S-EPMC7263443 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature