UltraPrep is a scalable, cost-effective, bead-based method for purifying cell-free DNA.

ABSTRACT: UltraPrep is an open-source, two-step method for purification of cell-free DNA that entails extraction of total DNA followed by size-selective enrichment of the smaller fragments that are characteristic of DNA originating from fragmentation between nucleosome. The advantages of the two related protocols that are described are that they can easily accommodate a wide range of sample input volumes, they rely on simple, magnetic bead-based technology, the yields of cfDNA are directly comparable to the most popular methods for cfDNA purification, and they dramatically reduce the cost of cfDNA isolation relative to currently available commercial methods. We provide a framework for physical and molecular quality analysis of purified cfDNA and demonstrate that the cfDNA generated by UltraPrep meet

SUBMITTER: Raymond CK 

PROVIDER: S-EPMC7263608 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature