MiR-877-5p Suppresses Gastric Cancer Cell Proliferation Through Targeting FOXM1.

ABSTRACT:

Purpose

miR-877-5p has been reported as a tumor suppressor in multiple cancers. Its role in gastric cancer, however, remains unclear. Hence, the purpose of this study was to elucidate the function, and underlying molecular mechanism, of miR-877-5p in the development of gastric cancer.

Materials and methods

We first analyzed miR-877-5p expression using the Gene Expression Omnibus (GEO) database and detected its expression in gastric cancer and gastric epithelial cells via real-time quantitative PCR (qRT-PCR). We then assessed the role of miR-877-5p in gastric cancer proliferation, apoptosis, and cell cycling. The gene targeted by miR-877-5p was predicted by bioinformatic analysis and confirmed by dual luciferase assay. Subsequently, rescue assays were carried out to validate

SUBMITTER: Wu K 

PROVIDER: S-EPMC7263828 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature