Porcine parvovirus nonstructural protein NS1 activates NF-κB and it involves TLR2 signaling pathway.

ABSTRACT: BACKGROUND:Porcine parvovirus (PPV) is a single-stranded DNA virus that causes porcine reproductive failure. It is of critical importance to study PPV pathogenesis for the prevention and control of the disease. NS1, a PPV non-structural protein, is participated in viral DNA replication, transcriptional regulation, and cytotoxicity. Our previous research showed that PPV can activate nuclear factor kappa B (NF-κB) signaling pathway and then up-regulate the expression of interleukin (IL)-6. OBJECTIVES:Herein, the purpose of this study is to determine whether the non-structural protein NS1 of PPV also has the same function. METHODS:Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay, western blot, immunofluorescence assay and smal

SUBMITTER: Jin X 

PROVIDER: S-EPMC7263913 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature