CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro.

ABSTRACT:

Objective and design

CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel-cell adhesion, and regulates the expression of genes associated with leukocyte migration.

Material

Human THP-1, RAW264.7 and HEK293 cells.

Methods

Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells.

Results

We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar.

Conclusion

Taken together, the findi

SUBMITTER: Chan TW 

PROVIDER: S-EPMC7263946 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature