Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.

ABSTRACT: The COVID-19 pandemic is an unprecedented global challenge, and point-of-care diagnostic classifiers are urgently required. Here, we present a platform for ultra-high-throughput serum and plasma proteomics that builds on ISO13485 standardization to facilitate simple implementation in regulated clinical laboratories. Our low-cost workflow handles up to 180 samples per day, enables high precision quantification, and reduces batch effects for large-scale and longitudinal studies. We use our platform on samples collected from a cohort of early hospitalized cases of the SARS-CoV-2 pandemic and identify 27 potential biomarkers that are differentially expressed depending on the WHO severity grade of COVID-19. They include complement factors, the coagulation system, inflammation modulators, and pr

SUBMITTER: Messner CB 

PROVIDER: S-EPMC7264033 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature