On the Aggregation and Nucleation Mechanism of the Monoclonal Antibody Anti-CD20 Near Liquid-Liquid Phase Separation (LLPS).

ABSTRACT: The crystallization of Anti-CD20, a full-length monoclonal antibody, has been studied in the PEG400/Na₂SO₄/Water system near Liquid-Liquid Phase Separation (LLPS) conditions by both sitting-drop vapour diffusion and batch methods. In order to understand the Anti-CD20 crystallization propensity in the solvent system of different compositions, we investigated some measurable parameters, normally used to assess protein conformational and colloidal stability in solution, with the aim to understand the aggregation mechanism of this complex biomacromolecule. We propose that under crystallization conditions a minor population of specifically aggregated protein molecules are present. While this minor species hardly contributes to the measured average solution behaviour, it in

SUBMITTER: Pantuso E 

PROVIDER: S-EPMC7264149 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature