Dynamics of the 4D genome during in vivo lineage specification and differentiation.

ABSTRACT: Mammalian gene expression patterns are controlled by regulatory elements, which interact within topologically associating domains (TADs). The relationship between activation of regulatory elements, formation of structural chromatin interactions and gene expression during development is unclear. Here, we present Tiled-C, a low-input chromosome conformation capture (3C) technique. We use this approach to study chromatin architecture at high spatial and temporal resolution through in vivo mouse erythroid differentiation. Integrated analysis of chromatin accessibility and single-cell expression data shows that regulatory elements gradually become accessible within pre-existing TADs during early differentiation. This is followed by structural re-organization within the TAD and formation of spec

SUBMITTER: Oudelaar AM 

PROVIDER: S-EPMC7264236 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature