HDO production from [²H₇]glucose Quantitatively Identifies Warburg Metabolism.

ABSTRACT: Increased glucose uptake and aerobic glycolysis are striking features of many cancers. These features have led to many techniques for screening and diagnosis, but many are expensive, less feasible or have harmful side-effects. Here, we report a sensitive ¹H/²H NMR method to measure the kinetics of lactate isotopomer and HDO production using a deuterated tracer. To test this hypothesis, HUH-7 hepatocellular carcinoma and AML12 normal hepatocytes were incubated with [²H₇]glucose. ¹H/²H NMR data were recorded for cell media as a function of incubation time. The efflux rate of lactate-CH₃, lactate-CH₂D and lactate-CHD₂ was calculated as 0.0033, 0.0071, and 0.0.012 µmol/10⁶cells/min res

SUBMITTER: Mahar R 

PROVIDER: S-EPMC7264272 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature