Partial proteolysis improves the identification of the extracellular segments of transmembrane proteins by surface biotinylation.

ABSTRACT: Transmembrane proteins (TMP) play a crucial role in several physiological processes. Despite their importance and diversity, only a few TMP structures have been determined by high-resolution protein structure characterization methods so far. Due to the low number of determined TMP structures, the parallel development of various bioinformatics and experimental methods was necessary for their topological characterization. The combination of these methods is a powerful approach in the determination of TMP topology as in the Constrained Consensus TOPology prediction. To support the prediction, we previously developed a high-throughput topology characterization method based on primary amino group-labelling that is still limited in identifying all TMPs and their extracellular segments on the sur

SUBMITTER: Lango T 

PROVIDER: S-EPMC7264363 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature