Heterologous Expression of Laccase From Lentinula edodes in Pichia pastoris and Its Application in Degrading Rape Straw.

ABSTRACT: Rape straw cannot be efficiently degraded and utilized by ruminants due to its severe lignification and complex cross-linked structure between fiber and lignin. The laccases can catalyze the inter-unit bond cleavage in lignin substrates. Therefore, this study investigated the recombinant laccase from Lentinula edodes (LeLac) and its application in degrading rape straw. The LeLac was expressed using Pichia pastoris. It had the maximum activity at 60°C and pH 3.0 using ABTS as substrate and at 50°C and pH 4.0 using o-tolidine as substrate. The LeLac exhibited preferential oxidation of ABTS and featured resistance to high temperature, but relatively poor thermal stability. The LeLac activity could be strengthened by Cu²⁺ in dose-dependent manners. The LeLac cou

SUBMITTER: Liu C 

PROVIDER: S-EPMC7264821 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature