Application of monoclonal antibodies in quantifying fungal growth dynamics during aerobic spoilage of silage.

ABSTRACT: Proliferation of filamentous fungi following ingress of oxygen to silage is an important cause of dry matter losses, resulting in significant waste. In addition, the production of mycotoxins by some filamentous fungi poses a risk to animal health through mycotoxicosis. Quantitative assessment of fungal growth in silage, through measurement of ergosterol content, colony-forming units or temperature increase is limiting in representing fungal growth dynamics during aerobic spoilage due to being deficient in either representing fungal biomass or being able to identify specific genera. Here, we conducted a controlled environment aerobic exposure experiment to test the efficacy of a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) to detect the proliferation of fungal biomass

SUBMITTER: Le Cocq K 

PROVIDER: S-EPMC7264882 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature