Rapid culture-based detection of Legionella pneumophila using isothermal microcalorimetry with an improved evaluation method.

ABSTRACT: The detection and quantification of Legionella pneumophila (responsible for legionnaire's disease) in water samples can be achieved by various methods. However, the culture-based ISO 11731:2017, which is based on counts of colony-forming units per ml (CFU·ml^-1 ) is still the gold standard for quantification of Legionella species (spp.). As a powerful alternative, we propose real-time monitoring of the growth of L. pneumophila using an isothermal microcalorimeter (IMC). Our results demonstrate that, depending on the initial concentration of L. pneumophila, detection times of 24-48 h can be reliably achieved. IMC may, therefore, be used as an early warning system for L. pneumophila contamination. By replacing only visual detection of growth by a thermal sensor, but otherwise maint

SUBMITTER: Fricke C 

PROVIDER: S-EPMC7264898 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature