Pseudomonas putida KT2440 is naturally endowed to withstand industrial-scale stress conditions.

ABSTRACT: Pseudomonas putida is recognized as a very promising strain for industrial application due to its high redox capacity and frequently observed tolerance towards organic solvents. In this research, we studied the metabolic and transcriptional response of P. putida KT2440 exposed to large-scale heterogeneous mixing conditions in the form of repeated glucose shortage. Cellular responses were mimicked in an experimental setup comprising a stirred tank reactor and a connected plug flow reactor. We deciphered that a stringent response-like transcriptional regulation programme is frequently induced, which seems to be linked to the intracellular pool of 3-hydroxyalkanoates (3-HA) that are known to serve as precursors for polyhydroxyalkanoates (PHA). To be precise, P. putida is endowed with a surviv

SUBMITTER: Ankenbauer A 

PROVIDER: S-EPMC7264900 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature