Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus.

ABSTRACT:

Background

Circulating microRNAs (miRNAs) are potential biomarkers for many diseases. However, they can originate from non-disease specific sources, such as blood cells, and compromise the investigations for miRNA biomarkers. While small extracellular vesicles (sEVs) have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery, the most suitable blood sample for sEV miRNA biomarker studies has not been defined.

Aim

To compare the miRNA profiles between matched serum and plasma sEV preparations to determine their suitability for biomarker studies.

Methods

Matched serum and plasma samples were obtained from 10 healthy controls and 10 patients with esophageal adenocarcinoma. sEV isolates were prepared from serum and plasma using ExoQuick

SUBMITTER: Chiam K 

PROVIDER: S-EPMC7265139 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature