Essential factors involved in the precise targeting and insertion of telomere-specific non-LTR retrotransposon, SART1Bm.

ABSTRACT: Telomere length maintenance is essential for most eukaryotes to ensure genome stability and integrity. A non-long terminal repeat (LTR) retrotransposon, SART1Bm, targets telomeric repeats (TTAGG)n of the silkworm Bombyx mori and is presumably involved in telomere length maintenance. However, how many telomeric repeats are required for its retrotransposition and how reverse transcription is initiated at the target site are not well understood. Here, using an ex vivo and trans-in vivo recombinant baculovirus retrotransposition system, we demonstrated that SART1Bm requires at least three (TTAGG) telomeric repeats and a longer poly(A) tail for its accurate retrotransposition. We found that SART1Bm retrotransposed only in the third (TTAGG) tract of three repeats and that the A residue of the (T

SUBMITTER: Nichuguti N 

PROVIDER: S-EPMC7265360 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature