Universal promoter scanning by Pol II during transcription initiation in Saccharomyces cerevisiae.

ABSTRACT:

Background

The majority of eukaryotic promoters utilize multiple transcription start sites (TSSs). How multiple TSSs are specified at individual promoters across eukaryotes is not understood for most species. In Saccharomyces cerevisiae, a pre-initiation complex (PIC) comprised of Pol II and conserved general transcription factors (GTFs) assembles and opens DNA upstream of TSSs. Evidence from model promoters indicates that the PIC scans from upstream to downstream to identify TSSs. Prior results suggest that TSS distributions at promoters where scanning occurs shift in a polar fashion upon alteration in Pol II catalytic activity or GTF function.

Results

To determine the extent of promoter scanning across promoter classes in S. cerevisiae, we perturb Pol II catalytic activity

SUBMITTER: Qiu C 

PROVIDER: S-EPMC7265651 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature