YTHDF2 destabilizes m⁶A-modified neural-specific RNAs to restrain differentiation in induced pluripotent stem cells.

ABSTRACT: N⁶-methyladenosine (m⁶A) is an abundant post-transcriptional modification that can impact RNA fate via interactions with m⁶A-specific RNA binding proteins. Despite accumulating evidence that m⁶A plays an important role in modulating pluripotency, the influence of m⁶A reader proteins in pluripotency is less clear. Here, we report that YTHDF2, an m⁶A reader associated with mRNA degradation, is highly expressed in induced pluripotent stem cells (iPSCs) and down-regulated during neural differentiation. Through RNA sequencing, we identified a group of m⁶A-modified transcripts associated with neural development that are directly regulated by YTDHF2. Depletion of YTHDF2 in iPSCs leads to stabilization of these tra

SUBMITTER: Heck AM 

PROVIDER: S-EPMC7266156 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature