Reciprocal monoallelic expression of ASAR lncRNA genes controls replication timing of human chromosome 6.

ABSTRACT: DNA replication occurs on mammalian chromosomes in a cell-type distinctive temporal order known as the replication timing program. We previously found that disruption of the noncanonical lncRNA genes ASAR6 and ASAR15 results in delayed replication timing and delayed mitotic chromosome condensation of human chromosomes 6 and 15, respectively. ASAR6 and ASAR15 display random monoallelic expression and display asynchronous replication between alleles that is coordinated with other random monoallelic genes on their respective chromosomes. Disruption of the expressed allele, but not the silent allele, of ASAR6 leads to delayed replication, activation of the previously silent alleles of linked monoallelic genes, and structural instability of human chromosome 6.

SUBMITTER: Heskett MB 

PROVIDER: S-EPMC7266157 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature