Deciphering MET-dependent modulation of global cellular responses to DNA damage by quantitative phosphoproteomics.

ABSTRACT: Increasing evidence suggests that interference with growth factor receptor tyrosine kinase (RTK) signaling can affect DNA damage response (DDR) networks, with a consequent impact on cellular responses to DNA-damaging agents widely used in cancer treatment. In that respect, the MET RTK is deregulated in abundance and/or activity in a variety of human tumors. Using two proteomic techniques, we explored how disrupting MET signaling modulates global cellular phosphorylation response to ionizing radiation (IR). Following an immunoaffinity-based phosphoproteomic discovery survey, we selected candidate phosphorylation sites for extensive characterization by targeted proteomics focusing on phosphorylation sites in both signaling networks. Several substrates of the DDR were confirmed to be modulate

SUBMITTER: Bensimon A 

PROVIDER: S-EPMC7266272 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature