Examination of CRISPR/Cas9 design tools and the effect of target site accessibility on Cas9 activity.

ABSTRACT:

New findings

What is the topic of this review? In this review, we analyse the performance of recently described tools for CRISPR/Cas9 guide RNA design, in particular, design tools that predict CRISPR/Cas9 activity. What advances does it highlight? Recently, many tools designed to predict CRISPR/Cas9 activity have been reported. However, the majority of these tools lack experimental validation. Our analyses indicate that these tools have poor predictive power. Our preliminary results suggest that target site accessibility should be considered in order to develop better guide RNA design tools with improved predictive power. The recent adaptation of the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system for targeted genome eng

SUBMITTER: Lee CM 

PROVIDER: S-EPMC7266697 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature