Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes.

ABSTRACT: CRISPR-Cas systems employ ribonucleoprotein complexes to identify nucleic acid targets with complementarity to bound CRISPR RNAs. Analyses of the high diversification of these effector complexes suggest that they can exhibit a wide spectrum of target requirements and binding affinities. Therefore, streamlined analysis techniques to study the interactions between nucleic acids and proteins are necessary to facilitate the characterization and comparison of CRISPR-Cas effector activities. Bio-layer Interferometry (BLI) is a technique that measures the interference pattern of white light that is reflected from a layer of biomolecules immobilized on the surface of a sensor tip (bio-layers) in real time and in solution. As streptavidin-coated sensors and biotinylated oligonucleotides are commerc

SUBMITTER: Muller-Esparza H 

PROVIDER: S-EPMC7266957 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature