Purification and immobilization of engineered glucose dehydrogenase: a new approach to producing gluconic acid from breadwaste.

ABSTRACT:

Background

Platform chemicals are essential to industrial processes. Used as starting materials for the manufacture of diverse products, their cheap availability and efficient sourcing are an industrial requirement. Increasing concerns about the depletion of natural resources and growing environmental consciousness have led to a focus on the economics and ecological viability of bio-based platform chemical production. Contemporary approaches include the use of immobilized enzymes that can be harnessed to produce high-value chemicals from waste.

Results

In this study, an engineered glucose dehydrogenase (GDH) was optimized for gluconic acid (GA) production. Sulfolobus solfataricus GDH was expressed in Escherichia coli. The K _m and V</

SUBMITTER: Karagoz P 

PROVIDER: S-EPMC7268246 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature