Cell density alters bacterial community structure in culture-enriched 16S rRNA gene microbiota profiling.

ABSTRACT:

Objective

Microbial community profiling using 16S rRNA gene has provided invaluable insights into diverse microbial communities. Recently a few studies have attempted to use 16S rRNA gene microbiota profiling in combination with the conventional culture methods to explore bacterial communities. In this "culture-enriched microbiota profiling" approach, microbes in a sample are cultured on solid media, and the resulting colonies are combined and subjected to 16S rRNA gene microbiota profiling. Here we investigated the effect of cell densities as determined by varying levels of sample dilution on the culture-enriched microbiota profiles using De Man, Rogosa and Sharpe (MRS) agar medium as a model system.

Results

Cecal samples collected from 10 healthy chickens were serially dil

SUBMITTER: Adhikari B 

PROVIDER: S-EPMC7268277 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature