Exploiting geometric similarity for statistical quantification of fluorescence spatial patterns in bacterial colonies.

ABSTRACT: BACKGROUND:Currently the combination of molecular tools, imaging techniques and analysis software offer the possibility of studying gene activity through the use of fluorescent reporters and infer its distribution within complex biological three-dimensional structures. For example, the use of Confocal Scanning Laser Microscopy (CSLM) is a regularly-used approach to visually inspect the spatial distribution of a fluorescent signal. Although a plethora of generalist imaging software is available to analyze experimental pictures, the development of tailor-made software for every specific problem is still the most straightforward approach to perform the best possible image analysis. In this manuscript, we focused on developing a simple methodology to satisfy one particular need: automated proc

SUBMITTER: Espeso DR 

PROVIDER: S-EPMC7268344 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature