DryMass: handling and analyzing quantitative phase microscopy images of spherical, cell-sized objects.

ABSTRACT: BACKGROUND:Quantitative phase imaging (QPI) is an established tool for the marker-free classification and quantitative characterization of biological samples. For spherical objects, such as cells in suspension, microgel beads, or liquid droplets, a single QPI image is sufficient to extract the radius and the average refractive index. This technique is invaluable, as it allows the characterization of large sample populations at high measurement rates. However, until now, no universal software existed that could perform this type of analysis. Besides the choice of imaging modality and the variety in imaging software, the main difficulty has been to automate the entire analysis pipeline from raw data to ensemble statistics. RESULTS:We present DryMass, a powerful tool for QPI that covers all r

SUBMITTER: Muller P 

PROVIDER: S-EPMC7268593 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature