Optimization of hydrogenobyrinic acid biosynthesis in Escherichia coli using multi-level metabolic engineering strategies.

ABSTRACT:

Background

Hydrogenobyrinic acid is a key intermediate of the de-novo aerobic biosynthesis pathway of vitamin B₁₂. The introduction of a heterologous de novo vitamin B₁₂ biosynthesis pathway in Escherichia coli offers an alternative approach for its production. Although E. coli avoids major limitations that currently faced by industrial producers of vitamin B₁₂, such as long growth cycles, the insufficient supply of hydrogenobyrinic acid restricts industrial vitamin B₁₂ production.

Results

By designing combinatorial ribosomal binding site libraries of the hemABCD genes in vivo, we found that their optimal relative translational initiation rates are 10:1:1:5. The transcriptional coordination of the uroporphyrinogen III biosynthetic

SUBMITTER: Jiang P 

PROVIDER: S-EPMC7268678 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature