Decreased nuclear Pten in neural stem cells contributes to deficits in neuronal maturation.

ABSTRACT:

Background

PTEN, a syndromic autism spectrum disorder (ASD) risk gene, is mutated in approximately 10% of macrocephalic ASD cases. Despite the described genetic association between PTEN and ASD and ensuing studies, we continue to have a limited understanding of how PTEN disruption drives ASD pathogenesis and maintenance.

Methods

We derived neural stem cells (NSCs) from the dentate gyrus (DG) of Pten^m3m4 mice, a model that recapitulates PTEN-ASD phenotypes. We subsequently characterized the expression of stemness factors, proliferation, and differentiation of neurons and glia in Pten^m3m4 NSCs using immunofluorescent and immunoblotting approaches. We also measured Creb phosphorylation by Western blot analysis and expression of Creb-regulated genes with qR

SUBMITTER: Kang SC 

PROVIDER: S-EPMC7268763 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature